| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Seasonal dynamics of active SAR11 ecotypes in the oligotrophic Northwest Mediterranean Sea
|
| Autores: |
Salter, Ian
Galand, Pierre E.
Fagervold, Sonja K.
Lebaron, Philippe
Obernosterer, Ingrid
Oliver, Matthew J.
Suzuki, Marcelino T.
Tricoire, Cyrielle
|
| Data: |
2015-02
|
| Ano: |
2015
|
| Resumo: |
A seven-year oceanographic time series in NW Mediterranean surface waters was combined with pyrosequencing of ribosomal RNA (16S rRNA) and ribosomal RNA gene copies (16S rDNA) to examine the environmental controls on SAR11 ecotype dynamics and potential activity. SAR11 diversity exhibited pronounced seasonal cycles remarkably similar to total bacterial diversity. The timing of diversity maxima was similar across narrow and broad phylogenetic clades and strongly associated with deep winter mixing. Diversity minima were associated with periods of stratification that were low in nutrients and phytoplankton biomass and characterised by intense phosphate limitation (turnover timeo5 h). We propose a conceptual framework in which physical mixing of the water column periodically resets SAR11 communities to a high diversity state and the seasonal evolution of phosphate limitation competitively excludes deeper-dwelling ecotypes to promote low diversity states dominated (>80%) by SAR11 Ia. A partial least squares (PLS) regression model was developed that could reliably predict sequence abundances of SAR11 ecotypes (Q(2) = 0.70) from measured environmental variables, of which mixed layer depth was quantitatively the most important. Comparison of clade-level SAR11 rRNA: rDNA signals with leucine incorporation enabled us to partially validate the use of these ratios as an in-situ activity measure. However, temporal trends in the activity of SAR11 ecotypes and their relationship to environmental variables were unclear. The strong and predictable temporal patterns observed in SAR11 sequence abundance was not linked to metabolic activity of different ecotypes at the phylogenetic and temporal resolution of our study.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
https://archimer.ifremer.fr/doc/00374/48560/48969.pdf
https://archimer.ifremer.fr/doc/00374/48560/48970.pdf
https://archimer.ifremer.fr/doc/00374/48560/48971.pdf
https://archimer.ifremer.fr/doc/00374/48560/48972.pdf
https://archimer.ifremer.fr/doc/00374/48560/48973.pdf
https://archimer.ifremer.fr/doc/00374/48560/48974.pdf
https://archimer.ifremer.fr/doc/00374/48560/48975.pdf
DOI:10.1038/ismej.2014.129
https://archimer.ifremer.fr/doc/00374/48560/
|
| Editor: |
Nature Publishing Group
|
| Formato: |
application/pdf
|
| Fonte: |
Isme Journal (1751-7362) (Nature Publishing Group), 2015-02 , Vol. 9 , N. 2 , P. 347-360
|
| Direitos: |
2015 International Society for Microbial Ecology All rights reserved
info:eu-repo/semantics/openAccess
restricted use
|
|
